Identification and regulation of fusA, the polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi.
نویسندگان
چکیده
Fusarins are a class of mycotoxins of the polyketide family produced by different Fusarium species, including the gibberellin-producing fungus Fusarium fujikuroi. Based on sequence comparisons between polyketide synthase (PKS) enzymes for fusarin production in other Fusarium strains, we have identified the F. fujikuroi orthologue, called fusA. The participation of fusA in fusarin biosynthesis was demonstrated by targeted mutagenesis. Fusarin production is transiently stimulated by nitrogen availability in this fungus, a regulation paralleled by the fusA mRNA levels in the cell. Illumination of the cultures results in a reduction of the fusarin content, an effect partially explained by a high sensitivity of these compounds to light. Mutants of the fusA gene exhibit no external phenotypic alterations, including morphology and conidiation, except for a lack of the characteristic yellow and/or orange pigmentation of fusarins. Moreover, the fusA mutants are less efficient than the wild type at degrading cellophane on agar cultures, a trait associated with pathogenesis functions in Fusarium oxysporum. The fusA mutants, however, are not affected in their capacities to grow on plant tissues.
منابع مشابه
Identification and regulation of fusA , the polyketide synthase gene 4 responsible for fusarin production in Fusarium fujikuroi 5 6 7 8
1 2 3 Identification and regulation of fusA, the polyketide synthase gene 4 responsible for fusarin production in Fusarium fujikuroi 5 6 7 8 Violeta Díaz-Sánchez, Javier Avalos and M. Carmen Limón* 9 10 Department of Genetics, Faculty of Biology, University of Seville, E-41012 Seville, Spain. 11 12 13 Running title: Fusarin production in F. fujikuroi 14 15 16 17 *Corresponding author. Mailing a...
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 78 20 شماره
صفحات -
تاریخ انتشار 2012